The Laboratory Xenopus sp. (Laboratory Animal Pocket Reference)

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Product Description

Even though Xenopus is one of the two most popular non-mammalian animals used in biomedical research, its value in the lab suffers from a lack of standardization regarding their optimal care, breeding, and housing. Filling the need for such a reference, The Laboratory Xenopus sp provides researchers and lab managers with a practical, step-by-step manual that emphasizes the humane care and use of captive clawed frogs in basic as well as biomedical, and toxicological research. The Only Book of Its Kind Available to Researchers Amply illustrated with 50 color illustrations of management practices and technical procedures, this how-to guide: Offers quick reference on the humane care and use of clawed frogs in the laboratory Illustrates management practices and technical procedures with figures and tables Provides sources of additional information on frogs, feed, and sanitation supplies Supported with hypothesis-driven research, this well-organized manual explores the full range of responsibilities facing individuals who work with this species. The content is divided into intentionally brief sections that allow for the quick retrieval of essential information regarding important biological features and experimental methodology, as well as compliance and veterinary care, husbandry, housing, and water quality management. The book has an accompanying website with more information, including interesting frog trivia.

The Laboratory Xenopus sp. (Laboratory Animal Pocket Reference) Review

Xenopus laevis and Xenopus tropicalis have become increasingly important non-mammalian laboratory animal models in embryology, toxicology, cellular biology, physiology, biochemistry, and biomedical research studies. This book on Xenopus sp represents one of the latest volumes in the Laboratory Animal Pocket Reference Series. Like other titles in this series, this book contains chapters covering the important biological features, husbandry, management, veterinary care, and common experimental methods relating to Xenopus sp. in research laboratories. In addition, the last chapter provides additional information resources that supplement the necessarily brief summaries provided in a pocket reference. This book succeeds admirably in providing a convenient and comprehensive source of information addressing the unique characteristics and needs of Xenopus sp in laboratory settings. Its intended audience is research scientists, technicians, animal caretakers, and laboratory animal veterinarians, but the handbook would also serve as an excellent reference for pet owners. The first chapter, rich in illustrations, contains interesting and useful information regarding the origin, behavior, anatomy, and physiology of Xenopus. The second chapter focuses on the husbandry of Xenopus, addressing the critical macro and microenvironmental needs of these frogs by summarizing various housing options and providing detailed instructions on how to establish and maintain water quality. Other husbandry issues include the nutritional needs, sanitation, environmental enrichment, identification, transportation, and record keeping. A very brief third chapter contains miscellaneous information outlining some pertinent animal welfare regulations and resources, and outlines the potential zoonotic disease risks that may be associated with the use of Xenopus. Chapter 4 is of particular value for laboratory animal veterinarians and veterinary technicians, as it addresses critically important issues involving the quarantine and physical examination of Xenopus, as well as the diagnosis and management of important infectious diseases and other common disease conditions. Especially valuable is the detailed information (including drug dosages and preparations) on appropriate methods for anesthesia, aseptic surgery, analgesia, post-operative care, and euthanasia. Chapter 5 presents a practical guide for veterinarians, investigators and animal caretakers in methods for safely handling Xenopus. This chapter is enriched with high quality illustrations that show proper methods for restraint, drug injections, gross necropsy, and blood collection. Important and widely used methods used to induce ovulation and collect eggs/oocytes from adult females are described in detail and the reasons for the presently limited usefulness of hematology and clinical chemistries in Xenopus sp. is addressed. The final chapter (Resources) provides in one convenient location the organizations, internet resources, books, and vendors that can be consulted to supplement the information provided in this handbook. In all chapters, relevant and updated references are provided for further reading.
In summary, this much needed book addresses the unique characteristics and needs of Xenopus sp in laboratory settings, providing a wealth of information that is hard to find in one place. The book is comprehensive, easy to read and has complete and current references. It should be in the library of every veterinarian and researcher with interest in the biology, breeding, care, use, and common diseases of Xenopus sp.

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